Fig. 5
From: Systematic identification of Ctr9 regulome in ERα-positive breast cancer
Overlapped and distinct roles of PAF1 and Ctr9 in RNA polymerase II chromatin association. a Schematic representation of the number of genes with differential RNAPII binding in PAF1 knockdown MCF7 cells (shPAF1) as compared to control cells (shScr). b Metagene analysis showing total RNAPII occupancy measured by ChIP-seq in cells transduced with shScr (control) or shPAF1. All RNAPII-bound genes were normalized to same length with 2 kb extended upstream from TSS and 2 kb downstream from TTS for analyzing average density. TSS, transcription start site; TTS, transcription terminal sites. c Heatmap showing RNAPII occupancy at all genes in PAF1 knockdown (shPAF1) and control (shScr) conditions. d Venn diagram showing the overlap between genes with RNAPII binding decreased by PAF1 knockdown and genes with RNAPII binding decreased by Ctr9 knockdown. e Venn diagram showing the overlap between genes with RNAPII binding increased by PAF1 knockdown and genes with RNAPII binding increased by Ctr9 knockdown. f Representative genome browser views of RNAPII ChIP-seq profiles at IGFBP4 and GREB1 genes in the indicated conditions