Fig. 3

Analysis of CORALINA gRNAs. Next generation sequencing of CORALINA libraries have been used to analyze generated libraries. a, b Quantification of protospacer length of human (a, L1) and mouse (b, “pooled”) gRNA libraries. Protospacers are mostly between 18 and 36 bp long. Shown are protospacer lengths detected by forward (black) and reverse (grey) sequencing reads. c, d Proportions of human (c) and mouse (d) CORALINA gRNA protospacer aligning to genomic sites containing functional PAMs of published Cas9 proteins. e, f Distribution of sequenced gRNA protospacer alignments to the human (e) or mouse (f) genome. While the number of associated CORALINA gRNAs (black bars) is overall correlating to the size of the respective human chromosome (white bars), GC- and gene-rich chromosomes (grey bars, 16, 17, 19) are overrepresented relative to their size