Fig. 5
From: CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening
Analysis of CORALINA libraries. Bowtie has been used to classify targeting sites of gRNAs derived from human (L1, a) or mouse (“pooled”, b) CORALINA libraries. Pie charts indicating relative proportion of functional domains bound by gRNAs (middle). gRNA protospacer aligning to coding gene units (left) or repeats (right) are further sub-classified. Promoters are defined here as genomic sequences between 10 kb upstream to the transcriptional start site. Coding and noncoding gene and repeat information has been derived from UCSC. Numbers next to the sectors depict the median number of genomic alignments for the selected group of gRNAs. c Depiction of average specificity score of CORALINA libraries calculated according to [45] and compared to available gRNAs (Addgene) and a published gRNA library [49]. Error bars depict the standard error of the mean