Fig. 6

The schematic diagram of the pathway and temporal expressional patterns of lipid metabolism. The enzymes identified from functional unigenes annotation were used to produce the schematic diagram. The icons close to each enzyme show the results of DESeq analysis [10–80 DAF (left), 80–140 DAF (middle), and 140–170 DAF (right); red: up-regulation; yellow: no significant difference expression; green: down-regulation]. The identified key enzymes involved in lipid metabolism include acetyl-CoA carboxylase carboxyl transferase, (ACCase, EC:6.4.1.2); Malonyl-CoA-ACP transacylase, (MAT, EC:2.3.1.39); 3-Ketoacyl ACP synthase II, (KASII, EC:2.3.1.179); 3-Ketoacyl ACP synthase III, (KAS III, EC: 2.3.1.180); 3-Ketoacyl ACP reductase, (KAR, EC:1.1.1.100); 3R-hydroxymyristoyl ACP dehydrase, (HAD, EC:4.2.1.-); enoyl-ACPreductase I, (EAR, EC:1.3.1.9); fatty acyl-ACP thioesterase A, (FATA, EC:3.1.2.14); fatty acyl-ACP thioesterase B, (FATB, EC:3.1.2.14 3.1.2.21); acyl-ACP desaturase, (SAD, EC:1.14.19.1); palmitoyl-CoA hydrolase, (PCH, EC:3.1.2.2); long-chain acyl-CoA synthetase, (ACSL, EC:6.2.1.3); D12(v6)-Desaturase, (FAD2/6, EC:1.14.19.6); D15(v3)-Desaturase, (FAD8, EC:1.14.19.35); glycerol kinase, (GK, EC:2.7.1.30); glycerol-3-phosphate acyltransferase, (ATS1/GPAT, EC:2.3.1.15); lysophosphatidyl acyltransferase, (LPAAT, EC:2.3.1.51); phosphatidate phosphatase, (PP, EC:3.1.3.4); diacylglycerol O-acyltransferase 1, (DGAT1, EC:2.3.1.20); phospholipid: diacylglycerol acyltransferase 1, (PDAT1, EC:2.3.1.158); lysophosphatidylcholine acyltransferase, (LPCAT, EC:2.3.1.23 2.3.1.67) and phospholipase A2, (PLA2, EC:3.1.1.4). Lipid substrates are abbreviated: 16:0, palmitic acid; 18:0, stearic acid; 18:1, oleic acid; 18:2, linoleic acid and 18:3, linolenic acid