Fig. 6

RT-PCR testing of candidate testis-specifically spliced genes. Expression of the predicted testis-specific splice form was assessed using primers designed to span the predicted testis-specific exon-exon junction. Expression of other splice forms was assessed using additional primers targeting either multiple splice forms – both the predicted testis-specific splice form and other splice forms – but yielding products of different sizes (a), or other splice forms only (b). Note that primers amplifying splice forms other than the predicted testis-specific splice form may still yield a product in testis samples, as these splice forms may be expressed in the testis in addition to the testis-specific splice form. The splice forms illustrated here are simplified examples