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Table 1 8plex, 5plex and 9plex primers and respective cycling conditions. The gene name, primer sequence and amplicon size are reported. Lowercase letters in the gene sequences (8plex) represent the tails added in order to obtain the same annealing temperature

From: Comparative genomics of European avian pathogenic E. Coli (APEC)

8 PLEX
Gene name Sequence 5′–3′ Amplicon size bp
R1F + gcgaaaaGAGTAATGTCGGGGCATTCA 551
R1R + aggccaTTCCTGGCAAGAGAGATAAG
R2F + gcgaGATCGACGCCGGAATTTTTT 1141
R2R + gcgagaAGCTCCATCATCAAGTGAGA
R3F + agccaGGCCAAAACACTATCTCTCA 1785
R3R + agcgccGTGCCTAGTTTATACTTGAA
R4F + gcgcgcaTGCCATACTTTATTCATCA 699
R4R + gcgcTGGAATGATGTGGCGTTTAT
K12F + gcaagTTCGCCATTTCGTGCTACTT 916
k12R + acgcgcTAATCATAATTGGAATGCTGC
chuAF + aaatttgGACGAACCAACGGTCAGGAT 279
chuAR + atttagTGTGAAGTGTCAGGAGACGCTG
yjaAF + aaaaaaCCGCCAGTACCAGGGACA 211
yjaAR + gcagaaaaATGGAGAATGCGTTCCTCAA
TSPEF+ gcgaaaaaGAGTAATGTCGGGGCATTCA 152
TSPER+ aaggCGCGCCAACAAAGTATTACG
  1. Cycling conditions: 95 °C for 5 min (Initial denaturation), followed by 2 cycles 95 °C (denaturation) for 30s, 50 °C for 30s (annealing), and 72 °C for 60s followed by 33 cycles 95 °C for 30s (denaturation), 58 °C for 30 s (annealing), and 72 °C for 60s (polymerization). On completion of 35 cycles the, a final polymerization was performed at 72 °C for 420 s