Comparative genomics of European avian pathogenic E. Coli (APEC)

BMC Genomics

Table 2 8plex, 5plex and 9plex primers and respective cycling conditions. The gene name, primer sequence and amplicon size are reported. Lowercase letters in the gene sequences (8plex) represent the tails added in order to obtain the same annealing temperature

5 PLEX
Gene Name	Sequence 5′–3′	Amplicon size bp
iroN F	AATCCGGCAAAGAGACGAACCGCCT	553
iroN R	GTTCGGGCAACCCCTGCTTTGACTTT	553
ompT F	TCATCCCGGAAGCCTCCCTCACTACTAT	496
ompT R	TAGCGTTTGCTGCACTGGCTTCTGATAC	496
hlyF F	GGCCACAGTCGTTTAGGGTGCTTACC	450
hlyF R	GGCGGTTTAGGCATTCCGATACTCAG	450
Iss F	CAGCAACCCGAACCACTTGATG	323
Iss R	AGCATTGCCAGAGCGGCAGAA	323
iutA F	GGCTGGACATCATGGGAACTGG	302
iutA R	CGTCGGGAACGGGTAGAATCG	302

Cycling conditions: 95 °C for 5 min (Initial denaturation), followed by 35 cycles at 95 °C for 30s (denaturation), 55 °C for 30s (annealing), and 72 °C for 40s (polymerization). On completion of 35 cycles, a final polymerization was performed at 72 °C for 420 s

ISSN: 1471-2164