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Table 3 Digestive enzymes expressed in Olavius algarvensis

From: Transcriptomic and proteomic insights into innate immunity and adaptations to a symbiotic lifestyle in the gutless marine worm Olavius algarvensis

Transcript ID a)

Protein ID

Annotation

Consensus localization evidenceb)

Substrate or function

Protein digestion

comp310626_c3

n.d.

pancreatic carboxypeptidase A1

secreted

release of C-terminal amino acids

comp330196_c1

n.d.

pancreatic carboxypeptidase A2

secreted

release of C-terminal amino acids

comp209868_c0

n.d.

pancreatic carboxypeptidase A2

secreted

release of C-terminal amino acids

comp329532_c3

n.d.

pancreatic carboxypeptidase A2

secreted or membrane

release of C-terminal amino acids

comp328734_c12

n.d.

carboxypeptidase

secreted

peptides

comp328734_c1

n.d.

carboxypeptidase

secreted

peptides

comp328734_c4

n.d.

carboxypeptidase

secreted

peptides

comp326419_c0

n.d.

uncharacterized carboxypeptidase

secreted

peptides

comp330196_c2

n.d.

uncharacterized carboxypeptidase

secreted

peptides

comp319717_c3

n.d.

lysosomal Pro-X carboxypeptidase

lysosomal

proline - amino acid bonds

comp320275_c1

n.d.

chymotrypsin A

secreted

Tyr-/Trp-/Phe-/Leu-|-Xaa bonds

comp306409_c1

n.d.

chymotrypsin B

secreted

Tyr-/Trp-/Phe-/Leu-|-Xaa bonds

comp334148_c2

BF11_334148_c2_seq1_11 BF11_334148_c2_seq2_10

chymotrypsin-like protease ctrl-1

secreted

proteins

comp331491_c1

BF11_331491_c1_seq1_7

pancreatic elastase

secreted

proteins

comp334775_c2

n.d.

cathepsin B

secreted

Arg – Arg/–Xaa bonds

comp335560_c0

BF11_335560_c0_seq5_5 BF11_335560_c0_seq9_5

cathepsin B

lysosomal

Arg – Arg/–Xaa bonds

comp306522_c2

n.d.

cathepsin F

lysosomal

peptides, cleaves Phe/Leu

comp306522_c3

n.d.

cathepsin F

lysosomal

peptides, cleaves Phe/Leu

comp308536_c0

BF11_308536_c0_seq1_12

cathepsin F

lysosomal

peptides, cleaves Phe/Leu

comp283346_c1

n.d.

cathepsin L

lysosomal

proteins

comp306922_c1

BF11_306922_c1_seq1_34

cathepsin L

lysosomal

proteins

comp315575_c0

n.d.

cathepsin L

lysosomal

proteins

comp315575_c2

FD_315575_c2_seq1:64:270:1:+

cathepsin L

lysosomal

proteins

comp328653_c0

n.d.

cathepsin L

lysosomal

proteins

comp329800_c6

n.d.

cathepsin O

lysosomal

peptides (endopeptidase)

comp314408_c1

n.d.

cathepsin Z

lysosomal

C-terminal amino acids (not Pro)

comp308700_c0

n.d.

cathepsin C (dipeptidyl peptidase I)

lysosomal

release of an N-terminal dipeptide

comp329456_c1

n.d.

cathepsin C (dipeptidyl peptidase I)

lysosomal

release of an N-terminal dipeptide

comp328746_c4

n.d.

alpha amylase

secreted

α1- > 4 glycosidic bonds

comp324906_c1

BF11_324906_c1_seq1_9 BF11_324906_c1_seq2_12

alpha amylase

secreted or membrane

α1- > 4 glycosidic bonds

comp335205_c1

BF11_335205_c1_seq1_20

lysosomal alpha glucosidase

secreted or membrane

α1- > 4 glycosidic bonds

comp320084_c0

n.d.

lysosomal beta-mannosidase

lysosomal

cleaves terminal β-D-mannose

comp334411_c3

n.d.

sucrase-isomaltase

secreted or membrane

α1- > 6 glycosidic bonds

comp329957_c8

BF11_329957_c8_seq2_17

acid trehalase

secreted or membrane

trehalose - > glucose

comp335402_c7

n.d.

acid trehalase

secreted or membrane

trehalose - > glucose

Lipid degradation

comp22535_c0

n.d.

lysosomal acid lipase

secreted

hydrolyzes steryl esters

comp249291_c0

n.d.

lysozyme

secreted

peptidoglycan (glycosidic bonds)

comp250229_c0

BF11_250229_c0_seq1_15 BF11_250229_c0_seq2_17

peptidoglycan recognition protein

secreted

peptidoglycan (peptide bonds)

comp335695_c10

n.d.

peptidoglycan recognition protein

secreted or membrane

peptidoglycan (peptide bonds)

comp330541_c4

n.d.

peptidoglycan recognition protein

secreted

peptidoglycan (peptide bonds)

comp314994_c0

n.d.

peptidoglycan recognition protein

secreted or membrane

peptidoglycan (peptide bonds)

comp332570_c2

n.d.

peptidoglycan recognition protein

secreted or membrane

peptidoglycan (peptide bonds)

  1. a)Defined as Trinity components; see [13]
  2. b)Probable subcellular localization of proteins based on the results of TMHMM [117, 118], SignalP-4 [119], Phobius [120], TargetP [121], DISTILL [122], LocTree3 [123], BaCelLo [124], and iLoc-Animal [125]. Secreted – enters secretory pathway and is either excreted to the extracellular space or confined to non-cytoplasmic insides of intracellular compartments; membrane – predicted to be membrane integral; lysosomal – predicted to be targeted towards the lysosome. See Additional file 3: Table S14 for details on localization evidence