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Table 1 Genome sequencing and assembly

From: Comparative genomics to explore phylogenetic relationship, cryptic sexual potential and host specificity of Rhynchosporium species on grasses

Parameter R. commune (UK7) R.c. (AU2) R.commune (13-13) R. secalis R. agropyri R. ortho-sporum
Primary seq. data Illumina PE (GAIIx) Illumina PE (GAIIx) Illumina MP (GAIIx) 454 Illumina PE (GAIIx) Illumina PE (GAIIx) 454a Illumina PE (GAIIx) 454 Illumina PE (GAIIx) Illumina PE (GAIIx) Illumina PE (HiSeq) Illumina MP (HiSeq) Illumina PE (GAIIx)
No. of reads 18 Mio. 35 Mio. 6.5 Mio 2.37 Mio 32.1 Mio. 25.23 Mio. 2.21 Mio 34.7 Mio. 1.86 Mio. 34 Mio. 104 Mio. 26 Mio. 24 Mio. 38 Mio.
Read length 2 × 36 bp 2 × 151 bp, 270 bp dist. 2 × 151 bp, 3 kb dist. 377 bp 2 × 101 bp 2 × 76 bp 366.5 bp 2 × 101 bp 410 bp 2 × 101 bp, 300 bp dist. 2 × 100 bp, 180 bp dist. 2 × 151 bp, 270 bp dist. 2 × 151 bp, 3 kb dist. 2 × 101 bp
Total length 1.29 Gb 9.7 Gb 1.4 Gb 894 Mb 6.48 Gb 1.92 Gb 810 Mb 7.0 Gb 746 Mb 6.9 Gb 20.8 Gb 7.8 Gb 7.3 Gb 7.6 Gb
Nominal seq. depth 26x 194x 29x 18x 130x 25x 15x 140x 15x 137x 420x 156x 146x 150x
Assembly software ALLPATHS-LG + SSPACE Velvet Velvet + Newbler + Minimus2 Newbler ALLPATHS-LG + SSPACE Velvet
Total seq. length (LFI) 55.59 Mb 54.32 Mb 53.93 Mb 56.80 Mb 52.18 Mb 58.16 Mb
  1. a 0.5 run 20 kb PE library, 1.5 runs 0.8 kb library