Comparative genomics to explore phylogenetic relationship, cryptic sexual potential and host specificity of Rhynchosporium species on grasses

Table 1 Genome sequencing and assembly

Parameter	R. commune (UK7)				R.c. (AU2)	R.commune (13-13)		R. secalis		R. agropyri				R. ortho-sporum
Primary seq. data	Illumina PE (GAIIx)	Illumina PE (GAIIx)	Illumina MP (GAIIx)	454	Illumina PE (GAIIx)	Illumina PE (GAIIx)	454^a	Illumina PE (GAIIx)	454	Illumina PE (GAIIx)	Illumina PE (GAIIx)	Illumina PE (HiSeq)	Illumina MP (HiSeq)	Illumina PE (GAIIx)
No. of reads	18 Mio.	35 Mio.	6.5 Mio	2.37 Mio	32.1 Mio.	25.23 Mio.	2.21 Mio	34.7 Mio.	1.86 Mio.	34 Mio.	104 Mio.	26 Mio.	24 Mio.	38 Mio.
Read length	2 × 36 bp	2 × 151 bp, 270 bp dist.	2 × 151 bp, 3 kb dist.	377 bp	2 × 101 bp	2 × 76 bp	366.5 bp	2 × 101 bp	410 bp	2 × 101 bp, 300 bp dist.	2 × 100 bp, 180 bp dist.	2 × 151 bp, 270 bp dist.	2 × 151 bp, 3 kb dist.	2 × 101 bp
Total length	1.29 Gb	9.7 Gb	1.4 Gb	894 Mb	6.48 Gb	1.92 Gb	810 Mb	7.0 Gb	746 Mb	6.9 Gb	20.8 Gb	7.8 Gb	7.3 Gb	7.6 Gb
Nominal seq. depth	26x	194x	29x	18x	130x	25x	15x	140x	15x	137x	420x	156x	146x	150x
Assembly software	ALLPATHS-LG + SSPACE				Velvet	Velvet + Newbler + Minimus2		Newbler		ALLPATHS-LG + SSPACE				Velvet
Total seq. length (LFI)	55.59 Mb				54.32 Mb	53.93 Mb		56.80 Mb		52.18 Mb				58.16 Mb

ISSN: 1471-2164