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Table 4 PICRUSt showing predicted relative abundance of KEGG ortholog groups (Level 2 KOs)

From: Functional analysis for gut microbes of the brown tree frog (Polypedates megacephalus) in artificial hibernation

KEGG pathways NH frogs AH frogs
Fall Winter Spring 4 °C
Amino acid metabolism 9.169 ± 0.301a 9.222 ± 0.337a 9.084 ± 0.215a 10.855 ± 1.291b
Cell motility 4.297 ± 0.552a 4.338 ± 0.508a 4.674 ± 0.238a 3.258 ± 0.3b
Cellular processes and signaling 3.572 ± 0.107 3.64 ± 0.135 3.702 ± 0.123a 3.488 ± 0.102b
Circulatory system 0.005 ± 0.004 0.004 ± 0.003a 0.004 ± 0.005 0.025 ± 0.026b
Endocrine system 0.265 ± 0.018a 0.273 ± 0.026a 0.258 ± 0.02a 0.463 ± 0.196b
Environmental adaptation 0.165 ± 0.013a 0.169 ± 0.013a 0.172 ± 0.012a 0.149 ± 0.004b
Enzyme families 2.224 ± 0.059a 2.216 ± 0.052 2.155 ± 0.128 1.946 ± 0.391b
Folding, sorting and degradation 2.092 ± 0.107a 2.162 ± 0.173a 2.095 ± 0.142a 2.384 ± 0.065b
Genetic information processing 2.404 ± 0.081 2.472 ± 0.118 2.507 ± 0.121a 2.321 ± 0.072b
Immune system diseases 0.03 ± 0.006a,c 0.029 ± 0.006a,c 0.024 ± 0.005b 0.036 ± 0.007c
Infectious diseases 0.312 ± 0.013a 0.318 ± 0.016a 0.319 ± 0.031 0.394 ± 0.055b
Lipid metabolism 2.759 ± 0.107a 2.733 ± 0.128a 2.71 ± 0.112a 3.96 ± 1.373b
Membrane transport 14.685 ± 1.045a 14.072 ± 1.519a 14.645 ± 1.177a 9.828 ± 2.509b
Metabolism 2.492 ± 0.057a 2.505 ± 0.068a 2.492 ± 0.065a 2.616 ± 0.102b
Metabolism of other amino acids 1.479 ± 0.08a 1.463 ± 0.077a 1.442 ± 0.107a 2.042 ± 0.516b
Metabolism of terpenoids and polyketides 1.353 ± 0.115a 1.384 ± 0.142a 1.351 ± 0.134a 2.282 ± 0.884b
Nervous system 0.093 ± 0.003a 0.092 ± 0.003a 0.091 ± 0.003a 0.104 ± 0.008b
Poorly characterized 4.872 ± 0.083a 4.909 ± 0.15 4.912 ± 0.135 5.216 ± 0.403b
Signal transduction 2.15 ± 0.171a 2.194 ± 0.18a,b,d 2.302 ± 0.126b 1.926 ± 0.111c
Transcription 2.875 ± 0.159a 2.765 ± 0.248a 2.834 ± 0.262a 2.193 ± 0.342b
Xenobiotics biodegradation and metabolism 1.75 ± 0.152a 1.709 ± 0.177a 1.789 ± 0.306a 3.463 ± 2.176b
  1. Abbreviations: AH frogs artificially hibernating frogs, NH frogs, nonhibernating frogs. Values are means ± SD. Within each row, values not sharing superscripts (a, b, c, and d) differ significantly (p-value < 0.05, Wilcoxon’s test)