Fig. 4

Dependence of hybridization signal intensity on number and position of mismatches with respect to the probe sequence on the ATH1 array. a Values are arithmetic means (± SD; n = 8 to 94) of background-corrected raw probe signal intensity ratios for non-A. thaliana gDNA relative to A. thaliana gDNA hybridizations, shown as a function of the total number of mismatches of the heterologous target sequence compared to the corresponding A. thaliana 25-mer probe sequence. b Independence of hybridization signal intensity from the position of a single mismatch with respect to the probe sequence. Values are arithmetic means (± SD; n = 2 to 6) of background-corrected raw probe signal intensity ratios for non-A. thaliana gDNA relative to A. thaliana gDNA hybridizations, shown as a function of mismatch position in the heterologous target sequence compared to the corresponding A. thaliana probe sequence. Black circles represent the representative A. halleri reference dataset; white diamonds represent the representative A. lyrata reference dataset