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Table 3 Validation of predictions by assuming modular mode of binding and mutations using all 20 amino acids (Approach 3). Helices predicted for 9-bp repeating triplet of all 16GNN DNA targets are reported. The predictions are exact matches to the experimentally determined helices for the 16 GNN targets earlier reported by Qiang et al. 2002 [17]

From: Exploiting the recognition code for elucidating the mechanism of zinc finger protein-DNA interactions

Target DNA 5' - 3' Qiang et al. 2002 [17] F1 F2 F3
−11234567 −11234567 −11234567
GGG GGG GGG NSGELTE RSGTLTR RSGSLTR
GTA GTA GTA RSGDLTA NSGDLTR QSGTLTT
GGA GGA GGA RSGELTK NSGNLTR HSGTLTN
GCT GCT GCT HSGVLTR NSGTLTQ HSGSLTA
GGC GGC GGC RSGALTR RSGVLTT TSGHLTQ
GAG GAG GAG NSGTLTA TSGTLTR RSGTLTQ
GCG GCG GCG HSGDLTR RSGSLTR QSGSLTT
GAC GAC GAC HSGHLTR QSGTLTT QSGVLTR
GGT GGT GGT DSGNLTQ RSGALTA RSGSLTR
GTC GTC GTC HSGNLTQ HSGSLTR HSGELTQ
GCC GCC GCC TSGVLTT RSGTLTA RSGVLTK
GCA GCA GCA DSGSLTR RSGNLTK ASGVLTK
GTG GTG GTG NSGELTT RSGHLTT TSGSLTR
GAT GAT GAT HSGALTT NSGDLTR -
GTT GTT GTT NSGSLTK ASGHLTT TSGELTQ
GAA GAA GAA DSGNLTT DSGNLTT DSGNLTT