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Fig. 1 | BMC Genomics

Fig. 1

From: Genome-wide analysis of mRNAs associated with mouse peroxisomes

Fig. 1

Fractionation of organelles by centrifugation in OptiPrep density gradient. Eighteen fractions were collected from the OptiPrep density gradient and equivalent amounts of each fraction were analyzed by Western blot and qRT-PCR. a Western blot analysis of fractions using antibodies for different organelle protein markers: mitochondrial prohibitin, endosomal/lysosomal RAB7 and peroxisomal thiolase. b qRT-PCR validation analysis of fractions probing for mRNAs shown to be enriched in peroxisomal fraction by microarrays. Relative RNA levels are presented as percentage of RNA present in each fraction with 100% being the sum of RNA present in all fractions

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