Fig. 2
From: Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
Screening of MMEJ-enhancing factors using a fluorescence reporter assay. a Schematic diagram of MMEJ-dependent EGFP recovery. Green letters indicate chromophore sequence. Black line indicates CRISPR target sequence. Black box indicates protospacer adjacent motif (PAM) sequence. Black triangles indicate DSB site. MH, microhomology. CMV, cytomegalovirus promoter. b Schematic diagram of HEK293T-based reporter assay, followed by imaging analysis. c Fluorescence microscopy images of transfected cells. Bars, 500 μm. d Relative frequencies of MMEJ repair, fold to mock overexpression. The MMEJ frequencies were calculated by imaging analysis. Data are expressed as means ± SEM (n = 4)