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Fig. 2 | BMC Genomics

Fig. 2

From: Computer aided identification of a Hevein-like antimicrobial peptide of bell pepper leaves for biotechnological use

Fig. 2

Isolation of a peptide enriched fraction from bell pepper leaves using molecular exclusion chromatography (MEC). a MEC profile using a Protein Pack 60 Å column (7.8 × 300 mm, 5.0 μm, 2–10 kDa) of the fraction P1-RPC-C18; b Mass spectrometry profile of the fraction P4-MEC (arrow) showing the mono-charged (4241 Da) and the doubly charged (2122 Da) peptide-ions. The chromatography was performed in isocratic conditions using 25 mM Tris-HCl, pH 7.0, and 250 mM NaCl at 0.1 ml.min−1. Peaks were collected at 1.0 mL.min−1

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