Fig. 2

PCR amplifications of specific sites within the cassette for glyphosate resistant and susceptible A. palmeri. Lane 1: 1 kb ladder with band sizes of 10, 8, 6, 5, 4, 3, 2.5, 2, 1.5, 1, 0.7, 0.5, and 0.3 kb. Even lanes are the resistant A. palmeri and odd lanes are the sensitive. Lanes 2–3: primers binding within the putative HSC70 gene. Lanes 4–5: primers downstream of the HSC70. Lanes 6–7: primers upstream of the first putative rice sleeper gene. Lanes 8–9: primers within the putative rice sleeper gene. Lanes 10–11: primers immediately downstream of the first putative ricesleeper gene. Lanes 12–13: primers immediately upstream of EPSPS. Lanes 14–15: primers within EPSPS. Lanes 16–17: primers downstream of the second putative ricesleeper gene. Lanes 18–19: primers 20 kb upstream of the end of known cassette. Lane 20: negative control