Table 3 Genome size estimations based on flow cytometry of genotypes with known EPSPS cassette copies, and estimation of the size of the EPSPS cassette
Genotype | Sensitive (S) or Resistant (R) | Genome size estimated by FLOW (pg/2C) | EPSPS gene copy number (Relative to ALS) | % increase relative to S | Estimated genome size increase (based on ~ 300 kb cassette) (Mbp) | Estimated size of the EPSPS Cassette (Kbp) based on observed FLOW Data | Average of estimated sizes (±50%) |
|---|---|---|---|---|---|---|---|
PI632235 | S | 0.82 | 1 | N/A | N/A | N/A | |
P3 | R | 0.88 | 77.09 | 6.82 | 23 | 600 | |
P4 | R | 0.85 | 79.96 | 3.53 | 24 | 300 | 625 |
W3 | R | 0.87 | 92.66 | 5.75 | 28 | 500 | |
P1 | R | 0.93 | 106.62 | 11.83 | 32 | 1,100 |