Fig. 2

Absolute quantification of Ty1-copia retrotransposon families. The quantification was performed in 14 flax cultivars, based on amplification from their reverse transcriptase (RT) domains. The log10 of molecule copy number (mcn) was calculated using an online tool (see text) that accounts for plasmid+insert size. This value was used along Ct to generate standard curves to calculate molecule copy numbers for RTs, which were normalized to ETIF1 to find absolute copy number. Families depicted are: a RLC_Lu0, b RLC_Lu1, c RLC_Lu2, d RLC_Lu6, e RLC_Lu8, f RLC_Lu28. Error bars = standard deviation. Numbers above represent significant differences of the respective cultivar to other cultivars (Dunn’s multiple comparison test p ≤ 0.05) which are numbered as: 1. Adelie, 2. Aurore, 3. Belinka, 4. Bethune, 5. Blizzard, 6. Drakkar, 7. Evea, 8. Hermes, 9. Lutea, 10. Oleane, 11. Oliver, 12. rdf, 13. Stormont Cirrus. 14. Violin. The average copy number for all cultivars in each TE family is also indicated