Table 3 Primers used to detect the 93- and 388-kb inversions and the pTet plasmid
Primer target | Primer location | Primer name | Primer sequence (5´-3´) |
|---|---|---|---|
Inversion (93kb) | cj0143c | Cj-F1 | ATGCTTGAGGTGCTATACTGACAC |
Inversion (93kb) | cj0145 | Cj-R1 | CCTTATCCTTAAGCATAGCAGCAC |
Inversion (93kb) | cj0261c | Cj-F2 | ACCCCAGTTCCACATCCTATATC |
Inversion (93kb) | cj0263 (zupT) | Cj-R2 | TGGTAAATTGGCAAACTCACAG |
Inversion (388 kb) | cj0261c | CjInv5F | ACCCCAGTTCCACATCC |
Inversion (388 kb) | cj1562–1563 | CjInv5R | AACCCATCGACTTCATTTG |
Plasmid | cpp50 | cpp50tetOF1 | GAACTTTACTTGCACGGAATGGAG |
Plasmid | tetO | cpp50tetOR1 | GGCCTGGCGTATCTATAATGTTGA |
Plasmid | virB4 | virB4rcvapF1 | ATCTAGCTCATCATCATCTTCTGC |
Plasmid | vapD | virB4rcvapR1 | CTCGTCTTTCATCTATTGGTTCTT |