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Fig. 3 | BMC Genomics

Fig. 3

From: Dhurrin metabolism in the developing grain of Sorghum bicolor (L.) Moench investigated by metabolite profiling and novel clustering analyses of time-resolved transcriptomic data

Fig. 3

Cyanide potential in grains. nmol Hydrogen cyanide (HCN) released from macerated tissue at different developmental stages (a). The HCN release was measured from macerated tissue in buffer alone (Tissue, red bar), tissue in buffer plus 0.67 U/mL almond β-glucosidase (Tissue + BGD, orange bar) and tissue in buffer plus 0.67 U/mL almond β-glucosidase and an additional 5 nmol dhurrin (Tissue + BGD + Dhurrin, blue bar). For each sample/bar, the total possible release of HCN from dhurrin is indicated by the striped bar stacked on top of the measured HCN release. The total possible release of HCN is calculated from the equation describing the trendline for dhurrin in Fig. 1b. The HCN release was measured a second time with macerated tissue from 25 DAA with 5 U/mL almond β-glucosidase instead of 0.67 U/mL (b). As a control, an assay measuring HCN release from varying concentrations of dhurrin (1–16 nmol) in buffer was also performed (c)

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BMC Genomics

ISSN: 1471-2164

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