Table 1 A summary of all 21 attributes used in the project

Pepstats^a

Peptide properties: tiny, small, aliphatic, aromatic, polar, non-polar, charged, basic, acidic

Molecular weight

N₃₀ region

Charge

A₂₈₀ molar extinction coefficient

A₂₈₀ molar extinction coefficient (1 mg/ml)

Probability of expression in inclusion bodies (PEPIB)

Full length

N₃₀ region

N₃₀ region

N₃₀ region

N₃₀ region

CAI^a

Codon adaption index

Full length

ProtParam^b

Isoelectric point

Instability index

Aliphatic index

GRAVY Score

Full length

N₃₀ region

N₃₀ region

N₃₀ region

POODLE-S^c

N-terminal disorder (N₃₀ disorder)

N₃₀ region

This study

G + C content

Full length