Table 1 Details of primers used for RT-qPCR
Gene | Abbrev. | Sequence | Product length (bp) |
|---|---|---|---|
Piwi-Like RNA-Mediated Gene Silencing 1 | PIWIL1 | F:CTGGTTCTCTCGCTGTGTGT | 90 |
| Â | R:TTCCAAGCCCTTAGAGCAGC | ||
Spermatogenesis Associated 4 | SPATA4 | F:CTCTCGATCACCATCCTGCC | 106 |
| Â | R:CTCAATGCACTCATGCTCGC | ||
Inhibin beta A | INHBA | F:AGGTGGTGGATGCTCGAAAG | 125 |
| Â | R:GGTCTCCTGACACTGCTCAC | ||
Forkhead box O3 | FOXO3 | F:ATGGCAACCAGACACTCCAG | 97 |
| Â | R:CTGGCCTGAGACATCAAGGG | ||
Phosphatase and tensin homolog | PTEN | F:CGGCCGTTCCGAGGATT | 99 |
| Â | R:CTGGATGGTTGCAGCGACT | ||
Cytochrome P450, Family 51, Subfamily A, Polypeptide 1 | CYP51A1 | F:TACCTACCTGCTGGGGAGTG | 107 |
| Â | R:TCCCAAACACAGGTGTCGTC | ||
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | F:CTGCTGACGCTCCCATGTTTGT | 150 |
| Â | R:TAAGTCCCTCCACGATGCCAAA |