Fig. 4

Comparison of topological domain calling methods for different preprocessing method and sequencing depth. TAD calling methods were assessed in terms of boundary overlap between replicates (a), number of identified topological domains (b) and boundary overlap across replicates upon increasing sequencing depth (c) for different matrix preprocessing (filtered and IC corrected) and different sequencing depths (10 million, 20 million and 40 million reads). For TAD calling, only the optimal caller/parameter value pairs are shown (defined as the ones achieving the maximum boundary overlap for IC and 40 million reads). The boxplot and line colors correspond to the different TAD callers