Fig. 4

Identification and confirmation of small intragenic RNAs. The intraRNA (SR0921) was confirmed via Northern blot analyses for intra-bbq07. a The deep sequencing results are displayed in a coverage map as described in Fig. 2, except the yellow box indicates the region called as a small intraRNA by our peak finder. Putative ORFs are indicated by black lines with the canonical start codon (AUG) illustrated by a green box, the non-canonical start codon illustrated by the green box with a black outline, and stop codons indicated by a red box. b Northern blot analyses of total RNA fractionated on a denaturing polyacrylamide gel, blotted to a nylon membrane, and hybridized with oligonucleotides. The predicted transcripts are denoted and marked with the appropriate band (* for sRNA) in the Northern blot. Two independent experiments were performed and representative data are shown