Fig. 5
From: Temperature-dependent sRNA transcriptome of the Lyme disease spirochete
Identification and verification of intergenic small RNAs. IG-sRNAs (SR0961 and SR0897) were confirmed by Northern blot analyses. a and c The deep-sequencing results for the IG-sRNAs between bbb13 and bbb14 and between bba34 and bba36 are displayed in a coverage map as described in Fig. 2. A putative ORF is indicated by a black line with a canonical start codon (AUG) illustrated by a green box and the stop codon indicated by a red box. b and d Northern blot analyses of total RNA fractionated on a denaturing polyacrylamide gel, blotted to a nylon membrane, and hybridized with oligonucleotides. The predicted transcripts are denoted and marked with the appropriate band (*,^ for sRNA) in the Northern blot. Two independent experiments were performed and representative data are shown