Fig. 2

Validation of sequencing data by qRT-PCR for Foxn1 (a), Mmp-13 (b) and Mmp-9 (c). Histological (d-g), immunohistochemical (h-o) analyses of skin tissues from E14 (d, h, l), E18 (e, i, m), nude (f, j, n) and B6 (g, k, o) skin. Hematoxylin and eosin stained sections (d-g), and immunohistochemical detection of Mmp-9 protein expression (h-o). Figure l-o are the higher magnifications of figures h-k. Arrows indicate positive reaction for Mmp-9 in epidermis: E14 (h, l) and B6 (k, o); dermis: E18 (i, m) and subcutenous tissues in nude (j, n). Immunohistochemical sections (h-o) were counterstained with hematoxylin. E - epidermis. Scale bar = 50 μm (d-j), 100 μm (k), 20 μm (l-o)