Fig. 2

Multivariate analysis based on gene expression profile data in LT muscle. a Principal component analysis (PCA) score plots distinguishing between the LT muscle of lambs fed concentrate (triangle), and supplemented with vitamin E in the treatment, (+). b Partial least squares-discriminant analysis (PLC-DA) based on gene expression profile data. c Important features identified by PLS-DA. The top 15 genes are ranked by VIP scores. The colored boxes on the right indicate the relative expression of the corresponding gene in each group under study. d A bar graph showing the top 25 genes correlating with diet (CON, VE). Variables with the same distance from 0 with similar positions are positively correlated. Those with the opposite direction are negatively correlated. The light blue bars indicate genes showing a negative correlation, and the light pink bars indicate those with a positive correlation with the given pattern of change of treatment