Organelle_PBA, a pipeline for assembling chloroplast and mitochondrial genomes from PacBio DNA sequencing data

BMC Genomics

Table 3 Summary of the A. thaliana mitochondrial genome assembly

Input reads	Reference	Mapped reads	% Bases mapped	Estimated depth (x)	Assembly size (bp)	Organelle completed^a
5000	Arabidopsis thaliana	101	8.88	4	27,294	NO
10,000		215	8.80	8	57,303	NO
50,000		1,006	8.63	37	156,177	NO
100,000		1,861	7.92	68	152,405	NO
163,448		3,046	7.92	111	177,810	NO
490,143		11,080	8.31	434	136,334	NO
817,099		21,099	8.72	829	150,873	NO

^aThe mitochondrial genome assembly was considered complete when the size difference compared to the reference was <100 nucleotides

ISSN: 1471-2164