Fig. 11
ZbHaa1 is required for the transcriptional activation of ZbCRS5 gene in copper stressed cells of Z. bailii. a Growth curves of Z. bailii IST302 (●,○) and of its derived deletion mutant Zbhaa1∆ (▲,△) cultivated in MM medium (filled symbols) or in the same medium supplemented with CuSO4 (empty symbols). CuSO4 was added after 1 h of growth in MM medium to a final concentration of 0.08 mM. Yeast cells were harvested before CuSO4 supplementation (Time point 0) or 1 h after CuSO4 addition (Time point 1). b Comparison of the transcript levels from ZbCRS5 in Z. bailii IST302 (dark bars) or in the derived deletion mutant Zbhaa1∆ (grey bars) in the absence of CuSO4 (Time point 0) and 1 h after CuSO4 supplementation (Time point 1). ZbACT1 mRNA level was used as an internal control. The mRNA level from Z. bailii IST302 gene ZbCRS5 in the absence of CuSO4 (Time point 0) was set as 1 and the transcript levels from this gene were calculated relative to this control. mRNA values shown are the mean of, at least, three independent experiments. Results were analysed by two-way ANOVA. (*) p-value below 0.05, when comparing the relative mRNA levels in each strain at a given time point