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Fig. 2 | BMC Genomics

Fig. 2

From: The Zygosaccharomyces bailii transcription factor Haa1 is required for acetic acid and copper stress responses suggesting subfunctionalization of the ancestral bifunctional protein Haa1/Cup2

Fig. 2

Expression of HAA1 homologues of Z. bailii IST302 and CLIB 213T or S. cerevisiae BY4741 from ScHAA1 natural promoter increases acetic acid tolerance of S. cerevisiae BY4741 and derived deletion mutant haa1∆. Growth curves of a S. cerevisiae BY4741 and b S. cerevisiae BY4741 haa1∆ mutant strain expressing the cloning vector pGREG506_noHIS3 (●) or the recombinant plasmids pG_ZbHAA1-IST (▲), pG_ZbHAA1-CL (○) and pG_ScHAA1 (□), in MM4 medium (pH 4.0) without uracil (to keep selective pressure over the recombinant strains), supplemented with 0, 60 or 75 mM of acetic acid. The growth curves are representative of at least three independent growth assays that produced similar results

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BMC Genomics

ISSN: 1471-2164

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