Fig. 9

ZbHaa1 is required for copper tolerance. Susceptibility to copper was compared in a S. cerevisiae BY4741 parental and derived deletion mutants haa1∆ and cup2∆, in cup2∆ expressing the recombinant plasmids pG_ZbHAA1-IST, pG_ScHAA1 or the cloning vector and in b Z. bailii IST302 parental and derived deletion mutant Zbhaa1∆ strains. c S. cerevisiae BY4741 parental and derived deletion mutants haa1∆ and cup2∆ were also compared in what concerns their susceptibility to acetic acid. Cell suspensions with an absorbance at 600 nm of 0.05 ± 0.005 (lane a) and subsequent dilutions of 1:5, 1:10 and 1:20 (lanes b, c and d, respectively) were spotted onto the surface of the appropriate agarized media, supplemented with appropriate concentrations of copper or acetic acid. The images depicted were taken after 3 days of incubation at 30 °C and are representative of at least three independent experiments