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Table 3 Primers used for Northern blot analysis (A), RT-PCR (B), and quantitative RT-PCR (C)

From: Identification of functional enolase genes of the silkworm Bombyx mori from public databases with a combination of dry and wet bench processes

Gene

Forward primer

Reverse primer

A

BmEno1

5'-TCATGGCAAGGGAGTTTTGA-3'

5'-AAATCAGCCAAGTGCTTGTA-3'

BmEno2 and BmEnoC

5'-ATCCGAACTCAAATCCTCAG-3'

5'-ATCGCATTTCCCGCTTTTTT-3'

B

BmEno1

5'-GTAATAAAATCAATCAAGGCTCG-3'

5'-TTAGACCGGTCTACGGAAGTTCT-3'

BmEno2

5'-TCCGGAGGATTTTCTGTGCC-3'

5'-GCGGAGATTAGTTTGAGTAA-3'

BmEnoY

5'-CCATGCAGGAGTTCATGATCCT-3'

5'-CTGGGTAGTCCTTGATGAAGGA-3'

BmActin

5'-TATCGCCGACAGGATGCAGAAGGA-3'

5'-TAGAAGCACTTGCGGTGAACGATG-3'

HsGAPDH

5'-GTCAGTGGTGGACCTGACCT-3'

5'-TGCTGTAGCCAAATTCGTTG3'

C

BmEno1

5'-AAGGTCAATCAGATCGGTAGCG-3'

5'-ACTACCAGGTCGGCAATAAAGG-3'

BmEno2

5'-GGAGCTAACGCGATTCTTTGTG-3'

5'-ACGCCTGCCATATCCGATATG-3'

BmEno2 and BmEnoC

5'-AATAAATGCTGCCGCCAGTG-3'

5'-GGCAAGTTTATCCGACGACATG-3'

RP49

5'-ACGGTTCCAACAAGAAGACC-3'

5'-AAGAGACACCATGAGCGATCTC-3'

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BMC Genomics

ISSN: 1471-2164

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