Table 3 Differentially expressed genes that are downregulated (p < 0.001) in high isoflavonoid cultivars: DE gene models were inspected manually using annotations from the soybean database (Phytozome), and the ‘GO’ annotations associated with their Arabidopsis homologs (TAIR); they were compiled into families for consideration based on function, and potential involvement in the phenylpropanoid pathway and as underlying factors for isoflavonoid content
Glyma ID | Log2 Fold Change | Â | Â | Predicted Cellular Compartment, Biological Processes and Molecular Functions | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
AC Colombe vs. | Conrad vs. | Gene Product Description | Arabidopsis Homolog | Cellular Compartment | Metabolism | Transport | Transcriptional Regulation | Stress Response | |||
AC Glengarry | Pagoda | AC Glengarry | Pagoda | ||||||||
Glyma.02G157000.1 | −1.31 | −1.39 | −1.92 | −1.98 | Inorganic H pyrophosphatase family protein | AT1G15690.2 | vacuolar membrane |  | regulation of apoplastic pH/auxin transport |  | light-enhanced/repressed by pollen |
Glyma.03G066800.1 | −0.64 | −0.84 | −1.08 | −1.26 | NAD(P)-linked oxidoreductase superfamily protein | AT2G37770.2 | chloroplast | NADPH-dependent aldo-keto reductase |  |  | upregulated with cold, salt, drought |
Glyma.04G151500.1 | −1.78 | −1.66 | −2.67 | −2.52 | SNF7 family protein | AT5G22950.1 | cytosol/endosome membrane |  | Vacuolar-sorting protein SNF7/protein binding |  |  |
Glyma.05G100100.1 | −5.09 | −5.34 | −4.99 | −5.22 | dsRNA-binding protein | AT2G28380.1 | nucleus/cytoplasm |  |  | production of miRNAs; silencing | defense response to virus |
Glyma.08G070000.1 | −2.47 | −2.38 | −2.17 | −2.05 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | AT2G36690.1 | cytoplasm | oxidoreductase activity; root / hypocotyl |  |  |  |
Glyma.09G279100.1 | −0.89 | −1.23 | −1.36 | −1.68 | cytochrome P450, family 71, subfamily B, polypeptide 34 | AT3G26300.1 | integral membrane | CYP71B34; specialized metabolism |  |  |  |
Glyma.14G058600.1 | −1.00 | −1.09 | −1.11 | −1.18 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | AT3G11150.1 | chloroplast | oxidoreductase activity |  |  |  |
Glyma.14G061400.1 | −0.93 | −0.77 | −0.92 | −0.76 | Nucleotide-diphospho-sugar transferases superfamily protein | AT3G55830.1 | integral membrane | Glycosyl-transferase family |  |  | cell adhesion in plant development |
Glyma.15G031400.1 | −8.68 | −9.73 | −6.69 | −7.72 | beta glucosidase 15 | AT2G44450.1 | golgi/cell wall/endomembrane | degradation of flavonol bisglycosides |  |  | B-glucosidase activity to abiotic stress |
Glyma.17G173200.1 | −3.34 | −3.15 | −2.85 | −2.60 | dihydroflavonol 4-reductase | AT5G42800.1 | ER | dihydroquercetin to leucocyanidin |  |  | response to UV-B or sucrose |
Glyma.U000400.1 | −2.83 | −2.47 | −5.51 | −5.05 | APRATAXIN-like/bHLH protein | AT5G01310.1 | nucleus | adenylylsulfate sulfohydrolase activity |  | DNA metabolism and repair | response to DNA damage |