Fig. 2

Genotypes for selected genetic constructs used in this study. a Strain MS1 in which the Pm promoter and the gene encoding XylS is inserted between the promoter and start codon of algD. b Strain MS2 in which a transposon expressing algC from PmG5 is inserted into PFLU2944 in an algC ⁻ derivative of MS1. c Map of the transposon TnMS11 used for mutagenesis in this study. d Strain HE230 in which the gene encoding XylS and the PmG5 promoter is inserted between the promoter and start codon of algC in SBW25mucA. Inactivation of mucA confers a high level expression from wild type PalgD. Relevant promoters, and the two restriction sites used for sequencing are displayed above each map-line. The alg-genes are coloured to match Fig. 1, other P. fluorescens genes flanking the genes of interest are coloured blue, and heterologous genes and elements are coloured green. I and O denote the minitransposon ends