Fig. 1
qRT-PCR of genes identified as differentially expressed by RNA-seq. Quantitative RT-PCR using rpl-32 as an expression control was performed on samples used for RNA-seq to confirm changes in expression. Additionally, qRT-PCR was also performed on a set of samples prepared independently from the samples used for RNA-seq to further validate expression changes. ΔΔCT values are relative to matched control samples. Similar changes in expression were seen in both the RNA-seq and independent samples. Error bars represent SEM