Fig. 2

Highly expressed miRNAs in gonadal fat. a Out of 1841 mapped miRNAs, 183 were expressed at over 100 counts per million reads in at least one of the twelve libraries. These miRNAs are termed “highly expressed” miRNAs. b Percent abundance of miRNAs in gonadal fat of FCG mice. Values represent mean ± SD of the twelve sequencing libraries. c, d Three individual miRNA sequencing libraries representing biological replicates of XX females fed a high fat diet are correlated with the pooled sequencing library composed of those same mice. Highly expressed gonadal fat miRNAs (c) are more correlated between the individual and pooled libraries than miRNAs that are expressed at a lower level (d). Values represent mean ± SD. r² and p-values were calculated using Pearson product–moment correlation