Fig. 1From: Medium throughput bisulfite sequencing for accurate detection of 5-methylcytosine and 5-hydroxymethylcytosineTargeted BS-Seq library preparation requires 3 consecutive PCR reactions. Three successive steps for preparation of targeted BS-Seq libraries include the initial amplification of the loci of interest, the addition of the CS1 and CS2 universal adaptors, and the inclusion of the P5 and P7 adaptors that allow for cluster formation on the flow cellBack to article page