Fig. 1

Histone modifications in growing and quiescent cells. a. TCA lysates were prepared from cells in log phase (Log), at the diauxic shift (DS), and in purified populations of quiescent (Q) and nonquiescent (NQ) cells isolated 7 and 14 days after culture inoculation. Western blots were probed with antibodies against histones and methylated histones. An empty lane between the DS samples and day-7 Q samples was removed from the image. b Western blots were probed with antibodies against H3K79me1, H3K79me2, and H3K79me3 in lysates from log cells and purified populations of Q and NQ cells isolated 3, 5, and 7 days after culture inoculation. c-e Lysates were prepared from log cells and purified Q cells isolated at 3, 5, and 7 days after culture inoculation and western blots were probed with (c) anti-Myc antibodies to detect Set1, Set2, and Dot1; (d) anti-Flag antibodies to detect H2B and monoubiquitinated H2B (H2Bub1); and (e) antibodies that recognize unmodified RNA polymerase II (Rpb3-Myc) and the serine 5 (CTD-Ser5P) and serine 2 (CTD-Ser2P) phosphorylated forms of the RNAP II CTD. Actin served as a loading control in all blots, and the images represent the results from a single time-course experiment. A representative Actin blot is shown in panel a