Fig. 6

Transcript, RNAP II, and H3 methylation profiles of individual genes during the development of quiescence. Log and Q cells isolated at 3, 5, and 7 days after culture inoculation were analyzed for transcript levels and Rpb3-Myc, H3K4me3, H3K36me3, and H3K79me3 occupancy at (a) PMA1, a log cell expressed gene; (b) XBP1, a Q cell expressed gene; and (c) BAP2, a gene expressed in both cell types. Transcript levels represent the change in RNA abundance relative to levels in log cells. Rpb3-Myc and H3 modification occupancies were determined at the TATA and 5’ or 3’ ORF regions of each gene. Relative occupancy represents the IP/Input at each position relative to IP/Input at TELV (Rpb3-Myc) or to H3 IP/Input (H3K4me3, H3K36me3, H3K79me3). The data represent the average with STD of 2 biological replicates