Fig. 4

Analysis of DEM (differentially expressed miRNAs) in 02428-4x compared to 02428-2x during pollen and embryo sac development. (a, c) The number of DEM at different pollen development stages (a) and embryo sac development stages (c) in 02428-4x; (b, d) Venn analysis of DEM during pollen (B) and embryo sac (d) development in 02428-4x. PMA, MA, SCP and BCP represent pre-meiotic interphase, meiosis and single microspore stage and bi-cellular pollen stage, respectively. MF, MM, FMF and EES represent megasporocyte formation stage, megasporocyte meiosis stage, functional megaspore formation stage and eight-nucleate embryo sac developing-stage, respectively