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Fig. 1 | BMC Genomics

Fig. 1

From: Single-cell mRNA isoform diversity in the mouse brain

Fig. 1

Full-length mRNA isoforms in single cells. a Offset from the median mapped position for ERCC transcripts (left) and endogenous genes (right). Histograms showing the distribution of offsets for 5′ and 3′ ends, and internal splice junctions, as percentage of the count for zero offset. Horizontal axis shows offset in base pairs (note that the axis has variable bin sizes). Red, the total number of events used to create each plot. b Magnification of the 5′ and 3′ end for both ERCC transcripts and transcripts mapping to genes. Note that the bin sizes are larger after ±10 bp. c Relative position of transcript ends for ERCC (left) and endogenous genes (right). d Isoforms observed for Mbp. Each black track is a separate isoform (not a separate UMI). Pink track, reads from Zeisel et al. [22], obtained using a 5′-specific RNA-seq method. The arrow points to a peak representing the transcript start site from this extended dataset. Blue tracks, UCSC gene models. e Average number of isoforms per gene, reads per UMI, UMI per gene and UMI per isoform. Error bars, standard deviation. f Distribution of isoform event types. Error bars show standard deviations

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