Fig. 1

Example of one SNP identified on each C3′H and HQT amplicons by HRM curve profiles (a) and confirmed by sequencing (b). Results were obtained from the amplification of the C3′H exon 2 with primers C3H-3F and C3H-3R and from the amplification of HQT exon 2 with primers HQT-2F and HQT-2R. Reference ecotype from Beja AC2 (red) and B2 (green) and C3 (blue) ecotypes from Spain and Moldavia, respectively. The melting curve profiles of the reference AC2 (red) is different from the individual B2 (green) and C3 (blue), on C3′H and HQT genes, respectively. Sequencing of a C3′H amplicon indicates, at position 581 (exon 2), a cytosine in the reference AC2 and a thymine in B2 individual. Sequencing of a HQT amplicon indicates, at position 499, an adenine in the reference AC2 and a guanine in C3 individual