Table 2 Quality assessment of the assemblies using Benchmarking Universal Single-Copy Orthologs (BUSCO) and Reads Mapped Back to the Transcriptome (RMBT) of themselves and the combined assembly
| Â | Seed 05 | Seed 07 | Seed 08 | Seed 09 | Leaf | Root | Stem | All tissues |
|---|---|---|---|---|---|---|---|---|
BUSCO (%): | ||||||||
 Completeness | 86 | 76 | 79 | 83 | 74 | 84 | 84 | 83 |
 Duplicated | 18 | 18 | 19 | 20 | 18 | 18 | 17 | 24 |
 Fragmented | 5 | 16 | 13 | 9 | 13 | 7 | 8 | 10 |
 Missing | 7 | 7 | 7 | 7 | 11 | 7 | 7 | 5 |
RMBT to themselves: | ||||||||
 % mapped | 86.1 | 96.3 | 87.6 | 86.6 | 88.8 | 85.6 | 86.2 | 90.9 |
 % properly paired | 75.9 | 87.5 | 76.6 | 75.9 | 81.0 | 76.8 | 77.9 | 80.1 |
 % singletons | 3.8 | 1.3 | 3.4 | 3.7 | 3.4 | 3.9 | 3.6 | 3.2 |
 Total bp | 16,523,554 | 17,276,866 | 17,753,884 | 17,041,720 | 13,759,496 | 23,850,824 | 14,396,240 | 120,602,584 |
RMBT to Full: | ||||||||
 % mapped | 95.5 | 96.3 | 96.0 | 95.4 | 97.2 | 94.5 | 97.4 | 95.9 |
 % properly paired | 86.2 | 87.5 | 87.4 | 86.3 | 89.9 | 86.2 | 89.0 | 87.3 |
 % singletons | 1.4 | 1.3 | 1.3 | 1.5 | 1.0 | 1.4 | 1.0 | 1.3 |
 Total bp | 16,523,554 | 17,276,866 | 17,753,884 | 17,041,720 | 13,759,496 | 23,850,824 | 14,396,240 | 120,602,584 |