RNA-seq and metabolomic analyses of Akt1-mediated muscle growth reveals regulation of regenerative pathways and changes in the muscle secretome

Table 1 Ingenuity analysis of canonical signaling pathways enriched by Akt1-mediated muscle growth

	Pathways	P	Ratio	z-score
Growth/Cell Cycle Regulation	Cyclins and Cell Cycle Regulation	2.38	0.31	3.64
	Integrin Signaling	4.83	0.30	2.78
	ILK Signaling	4.93	0.31	2.50
	Estrogen-mediated S-phase Entry	3.08	0.50	2.31
	Actin Cytoskeleton Signaling	4.22	0.28	2.06
	Mitotic Roles of Polo-like Kinase	3.02	0.35	2.00
	p70S6K Signaling	2.39	0.28	1.77
Cellular Immune Response	Fcγ Receptor-mediated Phagocytosis in Macrophages and Monocytes	9.02	0.46	4.42
	Dendritic Cell Maturation	5.19	0.32	4.24
	Leukocyte Extravasation Signaling	5.50	0.31	3.78
	Toll-like Receptor Signaling	4.19	0.38	3.77
	Role of Pattern Recognition Receptors in Bacteria & Viruses Recognition	4.02	0.32	3.40
	Production of Nitric Oxide & Reactive Oxygen Species in Macrophages	5.52	0.32	2.94
	TREM1 Signaling	6.64	0.45	2.65
	IL-8 Signaling	4.23	0.29	2.50
	NF-κB Signaling	4.15	0.30	2.38
	Tumoricidal Function of Hepatic Natural Killer Cells	2.51	0.46	2.12

P is reported as (-log (B-H p-value)). Ratio denotes the ratio of DEG specific to the pathway in this study divided by the total number of the genes in this pathway designated by IPA knowledge database. Z-score indicates a pathway with genes exhibition overall increased mRNA levels (positive value) or decreased mRNA levels (negative value)

ISSN: 1471-2164