Fig. 1From: The abundance of homoeologue transcripts is disrupted by hybridization and is partially restored by genome doubling in synthetic hexaploid wheatThe morphology and cytology of T. turgidum AS2255 (AABB), A. tauschii AS60 (DD), the allotriploid AS2255 × AS60 (ABD) and the derived allohexaploid (AABBDD). a Fluorescent in situ hybridization (FISH) analysis of the 21 univalents presents at meiosis metaphase I in the meiocyte of an allotriploid plant. The probe 6C6-3 hybridizing to the centromeres fluoresced green. Bar: 10 μm. b Allotriploid pollen mother cells comprise a mixture of dyads (green arrowheads) and tetrads (red arrowheads). c Multi-colour genomic in situ hybridization of a root tip mitotic cell from an allohexaploid plant, showing 2n = 6x = 42. d Sequential multi-colour FISH of a root tip mitotic cell from an allohexaploid plant, showing that chromosomes of the A, B and D genome were all represented on basis of probes pSc119.2 (green), pAs1 (red), and pTa71 (yellow). e Morphology of 120 day old plants of AS2255, AS60 and their derived allotriploid (F1) and allohexaploid (S1). f Leaf width and length of the first four leaves of the plants. Whiskers indicate SD (allotriploid: n = 7, AS2255, AS60 and allohexaploid: n = 12)Back to article page