Fig. 2

HBx ChIP-seq identifies direct target genes involved in endocytosis. a H4 acetylation on Rab1A, Rab2B, Rab5B, Srebp2 and Beclin 1 genes promoters assessed in independent ChIP experiments. Anti-AcH4 ChIPed chromatin from mock, HBV-wt and HBx-mt HBV replicating HepG2 cells and NTCP-HepG2 HBV-infected cells. Results are expressed as in Fig. 1b. b–d qPCR quantification of Rab1A, Rab2B Rab5B, Srebp2 and Beclin-1 mRNA levels in mock, HBV-wt and HBx-mt HepG2 cells replicating HBV (b) HBV-infected NTCP-HepG2 (c) and PHHs (d). Results are expressed as fold induction relative to the mock-transfected/infected controls (Ctl) after normalization to endogenous human β-actin mRNAs. Data represent means ± SD from at least three independent experiments performed in duplicate. P-values: * 0,02 ≤ P < 0,05; ** 0,01 ≤ P < 0.02; *** 0,005 ≤ P < 0.01