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Fig. 4 | BMC Genomics

Fig. 4

From: Genome-wide identification of direct HBx genomic targets

Fig. 4

ChIP-seq miRNA peak validation and HBx targeted miRNAs expression. a HBx occupancy on the putative promoter regions (−5000 + 1000 nt relative to position +1 of each miRNA in MirBase v18) of selected miRNAs (Additional file 1: Figure S1d) was validated in independent ChIP experiments. Cross-linked chromatin from mock or HBV-wt replicating HepG2 cells was immunoprecipitated with a specific anti-HBx antibody or relevant IgG controls, and then analyzed by real-time qPCR using specific primer pairs (see Additional file 1: Table S6). ChIP results are expressed as Fold Induction (FI) of the % of Input and the histograms show the mean from three independent experiments; bars indicate SD. b–c miRNA profiles were analyzed by real-time qPCR, and normalized with respect to RNU38, in mock, HBV-wt and HBx-mt replicating HepG2 cells (b) and by Taqman PCR-arrays in HBV infected (12 dpi) PHHs (c). Data represent means ± SD from at least three independent experiments performed in duplicate. d HBx recruitment impacts on H4 histone acetylation of neighboring chromatin promoters. Anti-AcH4 ChIPs were performed and analysed as in a). P-values: * 0,02 ≤ P < 0,05; ** 0,01 ≤ P < 0.02; *** 0,005 ≤ P < 0.01

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