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Fig. 6 | BMC Genomics

Fig. 6

From: Genome-wide identification of direct HBx genomic targets

Fig. 6

Direct targeting of HBV 3.5Kb/pgRNA by miR-224. a In silico analysis of the HBV genome, performed with the RNAhybrid tool on the Bielefeld University Bioinformatics Server, revealed the presence of several putative miR-224 seed sequences that are conserved across HBV genotypes. b Upper panels. Schematic representation of the putative seed sequences of HBV genome cloned on the 3′ UTR of the Renilla luciferase gene in the pRL-TK vector (Promega). Lower panels. HepG2 cells were transfected with the above indicated luciferase constructs together with 30nM of pre-miR-224 or its relative control. Luciferase activity was assayed 30h after transfection and expressed as fold induction over the control. All histograms represent the mean of three indipendent experiments each performed in duplicate; bars indicate S.D. P-values: * 0,02 ≤ P < 0,05; ** 0,01 ≤ P < 0.02; *** 0,005 ≤ P < 0.01. Grey stars, HBV genotype A; black stars, genotype A, C, D. c Proposed model for a differential modulation of miR-224 expression by HBx and inflammatory cytokines in ealrly phases of HBV infection and in HCC

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