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Fig. 6 | BMC Genomics

Fig. 6

From: Deep Illumina sequencing reveals conserved and novel microRNAs in grass carp in response to grass carp reovirus infection

Fig. 6

Confirmation of the RNA-seq data by using RT-qPCR. Five known and five novel miRNAs were randomly selected for RT-qPCR analysis and compared with the data obtained using RNA-seq. The relative expression levels of the miRNAs at different time points were calculated as the ratio of gene expression level (qPCR) or normalized TPM (RNA-seq) relative to that at 0 days (control) post-infection. The data are represented as mean ± standard deviation of three replicates for the qPCR analysis

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BMC Genomics

ISSN: 1471-2164

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