Fig. 1

Schematic representation of the development system of PCR-based IT markers specific for the chromosome 4VS of Haynaldia villosa and 8% non-denaturing poly-acrylamide gels electrophoresis of PCR product of IT marker CINAU687. a Schematic representation of the development system of PCR-based IT markers specific for the chromosome 4VS of Haynaldia villosa. b 8% non-denaturing poly-acrylamide gels electrophoresis of PCR product of IT marker CINAU687. Grey boxes represented the exons and grey lines represented the introns. Red line, light blue line, deep blue line and light green line showed the intron 7 region of gene fragment AEGTA27279 corresponding to the intron regions of the scaffold of IWGSC_CSS_4AL_scaff_7149580, IWGSC_CSS_4BS_scaff_4952162, IWGSC_CSS_4DS_scaff_2323695 and Hecate_SCF226, respectively). The base number of intron 7 in the gene fragment of subgenome AA, BB, DD and VV was 658 bp, 367 bp, 415 bp and 468 bp, respectively